| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Gretna Elite Academy GEA 07 NPL |
02077-0011B6U-0062 | Jacob Pease |
| A2 | Azzurri Soccer Club Azzurri Italia |
03065-0011B6U-0004 | Martin Alvarez |
| A3 | Sporting Nebraska FC Sporting Nebr B Gold 2007 |
03036-0011B6U-0053 | William Taylor |
| A4 | Kearney Soccer Club KSC Nebraska United 07/08 |
06043-0011B6U-0014 | Andrew Rahe |
| A5 | Omaha United Soccer Club Omaha United SC 2007B Black |
03133-0011B6U-0011 | Thierry Ndjike |
| A6 | Sporting Nebraska FC Sporting Nebr Platinum B 2008 |
03036-0011B5U-0067 | Nathan May |
| A7 | Sting Soccer Sting NE 07B Davis |
03137-0011B6U-0019 | Ryan Davis |
| A8 | Dreamers FC DFC Aztecs 07 NPL |
03134-0011B6U-1776 | Rick Sanchez |
| A9 | Lex Soccer Unifut Lex Manchester |
06130-0011B6U-2008 | Artemio Munoz |